Diagnosis and Follow-up of Paroxysmal Nocturnal Hemoglobinuria by Flow Cytometry
Paroxysmal Nocturnal Hemoglobinuria (PNH) is the name given to the disease caused due to an acquired genetic mutation in the PIG-A gene which results in the reduction or absence of membrane proteins of the affected hematopoietic cells.
This mutation affects proteins involved in the interaction with complement regulators (CD55 and CD59) .This loss of complement regulation is especially sensitive on red cells resulting on severe pathophysiology episodes of the disease by causing episodes of intravascular and extravascular hemolysis.
Flow cytometry is the method recommended by current clinical guidelines.By Marking membrane proteins of different hematopoietic series affected celular clones are detected ,thus allowing not only their diagnosis but also their follow-up and monitoring.
CYTOMETRY - Evolution in the diagnosis by immunophenotype of CLPD
Chronic B cell lymphoproliferative syndromes (BCLPD) comprise a heterogeneous group of hematologic tumors that affect mature B lymphocytes. Recently, they have begun to be used routinely in clinical laboratories, immunophenotypic techniques that allow the labeling of antibodies with different fluorochromes and provide a specific, sensitive and reproducible count for the different cellular subpopulations.
CITOMETRY - Prognostic markers and Minimum Residual Disease
Monoclonal gammopathies (MG) are a group of disorders or entities characterized by clonal proliferation of plasma cells (PC). The immunophenotypic study of PC by multiparametric flow cytometry contributes decisively in the differential diagnosis, prognosis and monitoring of MG.
CITOMETRY - Celiac disease and flux citometry
The disease affects 1% of the population, but is known, is very underdiagnosed because it is often asymptomatic and symptoms occur discontinuously and with a highly variable clinical spectrum. The cytometric study is based on the immunophenotyping identification and quantification of intraepithelial lymphocytes (IELs) of the duodenal mucous.
CITOMETRY - Digital Citometry in Clinics
Digital cytometry allows us a quick study of the interactions between different cell populations suspended in a minimal amount of sample.
CYTOMETRY – Determination of ZAP-70 Chronic Lymphatic Leukemia (LLC) is the most frequent leukemia in the western adult population. The median survival rate is 10 yearsand the young have a higher risk of mortality due to the disease. For this reason, the evaluation of predictive factors and treatments that permit good quality, long-term remissions is highly important.