Molecular Biology
MOLECULAR BIOLOGY - Apolipoprotein E genotype and Alzheimer's disease
Apolipoprotein E (ApoE) is a protein of 299 amino acids constituted by a single polypeptide chain. ApoE is involved in the transport of cholesterol and other lipids in various tissues. It is the major apolipoprotein expressed in brain tissue, mainly in neuroglia. Polymorphic variations ApoE locus act as inherited risk factors and genetic susceptibility affect Alzheimer's disease.
MOLECULAR BIOLOGY - Massive sequencing for antiretroviral resistance HIV-1
From February 2016, the massive sequencing will be used for the test of resistance to HIV-1 antiretroviral in Catlab. This method has several advantages; the resistances of the three regions (Pro, RT, INT) are determined in one integrated test; it automatically determines the subtype of HIV-1, and finally, the new test allows the detection of mutations in viral quasispecies that only represent 1% of the viral population, compared to 20% of the current test.
MOLECULAR BIOLOGY - HLA-B5701
The determination of the HLA-B * 5701 is the first pharmacogenetic test in the context of treatment of HIV-1. The haplotype HLA-B * 5701 significantly predicted the risk of hypersensitivity reaction to Abacavir (ABC), which is currently the antiretroviral reverse transcriptase inhibitor, preferentially used to treat HIV-1.
MOLECULAR BIOLOGY - Change of method in celiac disease molecular study
Celiac disease is a chronic inflammatory bowel disease that affects from 1 to 3% of the Western population. At the molecular level, is associated with the HLA genes. From December 2013 we incorporated a new equipment that allows us to make a complete study of the two genetic regions associated with celiac disease (HLA-DQA/HLA-DQB). This equipment is designed to work with reverse hybridization technique adapted to a microarray plate, and also allows us to determine if these alleles are in heterozygous or homozygous state.
MOLECULAR BIOLOGY - Method change for the molecular study of the HFE mutation gen, related with hereditary hemochromatosis
Hereditary hemochromatosis (HH) is a genetic disease of autosomal recessive transmission wich causes an alteration in iron metabolism.
MOLECULAR BIOLOGY –Phenotype and Genotype of HLA-B27 The presence of the antigen HLA-B27 has been closely associated for more than 30 years with Ankylosing Spondylitis.
MOLECULAR BIOLOGY - New molecular biology techniques. 2009
We believe it can be of your interest to know all the new techniques the lounge of Molecular Biology Catlab has put in operation in 2009.