Catlab Reports Bulletin
The Catlab Reports Bulletin is published monthly. Until the publication of the Catlab website, it was mailed electronically to our clients. It consists of a monograph related with laboratory topics. Each month it addresses a different area of Catlab; the topics can be more specific (concrete diagnostic techniques) or deal with more generic aspects (Quality, Management, …).
High Performance Liquid Chromatography-Mass Spectrometry
Recently, and in collaboration with the Hospital Pharmacy Service of the Hospital
Mutua Terrassa University, a high-efficiency chromatography analytical system in tandem with a mass spectrometry detector (HPLC-MS) has been incorporated into Catlab.
This combination of analytical techniques will allow our laboratory to have,the considered, gold-standard method in clinical laboratory, and thus have the ability to measure analytes with great sensitivity and specificity. The study of immunosuppressants will be the first to make use of this technique and new ones will be added later.
Changes in the reporting of antibiotic susceptibility in the reporting of
microbiology New interpretation of susceptibility categories S, I and
As of June 2022, the categories used to generate Microbiology culture sensitivity reports in Catlab have been modified in order to include the new recommendations of the European Committee for Antimicrobial Susceptibility Testing (EUCAST).
Thus the less problematic definitions Susceptible(S) and Resistant(R) remain, but the term Intermediate(I) disappears and the term Sensitive: Susceptible to Increased Exposure: S(EI) begins to be used. This new term refers to microorganisms where treatment will increase therapeutic success by increasing antibiotic exposure, dosage or concentration at the site of infection. At Catlab, the Microbiology department remains available to clarify any doubts.
Role of Monocytic subpopulations on the differential diagnosis of the Chronic myelomonocytic leukemia
Chronic myelomonocytic leukemia (CMML) is a clonal hemopathy characterized by persistent monocytosis in peripheral blood along with somatic mutations. In recent years, several studies have shown that specific variations in the percentage of monocytoid subpopulations can become a specific and rapid marker to differentiate CMML from other neoplasms or reactive monocytosis.
Catlab has internalized the study of monocytoid subpopulations, performed in the Cytometry department, to contribute to the differential diagnosis of this hematological neoplasm.
Microbiological diagnostic of sepsia
Early detection of this organ dysfunction is key to reducing complications that can ultimately lead to patient death. In order to reduce response time, at Catlab, two algorithms are used from positive blood culture,thus allowing positive results and antibiogram to be informed as soon as possible.
Information for patients: Genetic testing
Catlab's Genetics department has produced an informative note aimed at patients, which briefly but clearly explains what a genetic study consists of, which sample is suitable and which methods currently exist.
Likewise, patients will also find information related to result interpretation and the advantages of carrying out these studies.
Methodology to avoid Daratumumab interference on Proteinogram interpretation.
Multiple Myeloma (MM) is the second most common blood malignancy and accounts for 1% of all types of cancer. Among the new drugs used in the treatment of this pathology are monoclonal antibodies. Since the appearance of therapies with these biological drugs, such as Daratumumab as the first line of action. It has been found interference caused by the use of these monoclonal antibodies making laboratory tests a bit more challenging.
Below we explain how we solve this interference in Catlab to achieve a correct interpretation and evaluation of the results.
Hexanucleotide expansion in C9orf72 gene, associated with frontotemporal dementia and amyotrophic lateral sclerosis
Frontotemporal Dementia (FTD) is considered one of the most common forms of dementia in the population under the age of 65, with a prevalence of 10 cases per 100,000. Patients with DFT suffer a degeneration of the neurons in the frontal and temporal lobes that leads to changes in behavior and personality, deficits in executive functions and language deficiencies. The expansion of the C9orf72 gene is considered the most common hereditary genetic cause for both diseases. This article describes the study for this expansion at Catlab.
Etiological diagnosis of onychomycosis using molecular biology techniques
Onychomycosis is the infection of the nail caused by fungus. It is the most common nail infection worldwide. Most are caused by dermatophyte fungi. The diagnosis is based on microscopic observation and culture, techniques with many limitations such as low sensitivity or slowness of cultures. For these reasons at Catlab, the appropriateness to introduce molecular techniques that significantly increase sensitivity and shorten response time was assessed.
Results obtained in Catlab using the new "Prenatal screening protocol for congenital anomalies in Catalonia"
In year 2018 and later in 2021, the screening protocol for congenital anomalies was updated. The latest updates to the protocol include the determination of Cell-free fetal DNA in patients at intermediate and high risk. These new protocols have made it possible to extend the non-invasive study to intermediate-risk patients and to avoid invasive tests in high-risk patients in which Cell-free fetal DNA tested negative.
In Catlab between March and December 2021, 3038 first trimester screenings were performed. Applying the aforementioned protocols, the improvement in the detection of anomalies was shown as 2 alterations were detected in patients with intermediate risk using fetal DNA, as well as the decrease in the need for invasive tests by avoiding this intervention in 66 high-risk patients.
New Laboratory Information System(LIS) at Catlab.
The laboratory information system (LIS) has become a key element in all stages of an increasingly automated analytical process.
Thus, from the computer input of the request, application of algorithms, generation of reflex tests based on various variables to the final request sending to petitionary untis.
Due to requirement increase, Catlab has recently migrated to Modulab, a more powerful, robust and constantly developing LIS that provides Catlab with the necessary tools to meet the current analitycal demand.