Catlab Reports Bulletin

The Catlab Reports Bulletin is published monthly. Until the publication of the Catlab website, it was mailed electronically to our clients. It consists of a monograph related with laboratory topics. Each month it addresses a different area of Catlab; the topics can be more specific (concrete diagnostic techniques) or deal with more generic aspects (Quality, Management, …).

Bulletin Nº74 - October 2016

MOLECULAR BIOLOGY - Apolipoprotein E genotype and Alzheimer's disease
Apolipoprotein E (ApoE) is a protein of 299 amino acids constituted by a single polypeptide chain. ApoE is involved in the transport of cholesterol and other lipids in various tissues. It is the major apolipoprotein expressed in brain tissue, mainly in neuroglia. Polymorphic variations ApoE locus act as inherited risk factors and genetic susceptibility affect Alzheimer's disease.

Bulletin Nº73 - September 2016

URGENT CARE - Hemolysis index calculation. Applications in Catlab
One of the clinical laboratory objectives is to inform biological values with high accuracy. Therefore, it must be guaranteed that samples are biological free from interference, such as hemolysis. In Catlab, rates of hemolysis informed by the manufacturer have been incorporated into computer systems laboratory, so that before an analytical result is validated, it will check whether the degree of hemolysis of the sample exceeds the threshold beyond which the result may be affected.

Bulletin Nº72 - July 2016

QUALITY - Customer satisfaction measure
During the years 2012-2015, Catlab has made a plan for measuring customer satisfaction; surveys have been conducted to different groups of primary care CST, HUMT and ICS. Here are the results of 8 consensual questions related to perception and assessment of the laboratory.

Bulletin Nº71 - June 2016

MICROBIOLOGY - Urinary tract infection. New circuit in Catlab
Urinary tract infections are one of the most frequent infectious diseases. The diagnosis of UTI is based on three factors: the patient's symptoms, examination of the formed elements of urine and urine culture. To optimize the microbiological diagnosis of UTI, a retrospective study of all urine cultures obtained by spontaneous urination was performed to set breakpoints that would allow rule planting negative urine cultures and no significant bacteriuria with sensitivity ≥ 95%. From the results, it is proposed to make a screening urine culture, which exclude the cultivation of all urine samples obtained by spontaneous urination, which leucocyte esterase or nitrites are not detected.

Bulletin Nº70 - May 2016

QUALITY - Accreditation of clinical laboratories according to ISO 15189
UNE-EN ISO 15189: 2013 is an international standard that specifies requirements for the competence and quality of clinical laboratories. In particular, it recognizes the technical competence of the laboratory analysis of specific magnitudes. Applying strict quality requirements demanded by ISO 15189 accreditation affects all processes in the laboratory (preanalytical, analytical and postanalíticos) and has been a major effort, both by the entity as of our professionals.

Bulletin Nº69 - April 2016

HORMONES - Progesterone. New reference values.
Currently, in Catlab, measurement of serum progesterone is performed on the Cobas 602 8000_e Roche Diagnostic (Elecsys), by a chemoluminescence immunoassay method (ECLIA). In order to improve the quality of the results obtained in our laboratory, we will proceed to replace the current reagent by a new reagent which have been changed its rat monoclonal antibodies into sheep monoclonal antibodies with greater specificity.

Bulletin Nº68 - March 2016

IMMUNOLOGY - Review of Prenatal Screening in Catlab
Since September 2015, the first-trimester prenatal screening for Down syndrome and Edwards syndrome, is done with a new method and a new calculation program. A comparative between the previous program Lifecycle Perkin Elmer and the new Ssdwlab6 Roche was made satisfactorily. And minimum requirements for imprecision and inaccuracy for biochemical markers were established with the new method of electrochemiluminescence with optimum results.

Bulletin Nº67 - February 2016

Biochemistry - CKD-EPI
Chronic kidney disease (CKD) is generally defined as a set of heterogeneous diseases that affect renal structure and function. Since 2002, it published a set of guidelines on the evaluation, classification, and stratification of the ERC. Staging classification for risk, they have used different calculations and equations. The currently recommended by the new guidelines is the equation CKD-EPI creatinine.

Bulletin Nº66 - January 2016

HEMATOLOGY - Haemoglobinopathies studies
Review of the various alterations of hemoglobin and the different methods used to detect them in Catlab. Structural haemoglobinopathies are classified by the disturbance affecting the solubility, stability, affinity for oxygen or maintenance of the reduced iron. The thalassemias are classified according to the globin chain stops synthesized, and we also have the thalassemic haemoglobinopathies, which are mutations in the globin genes that cause structural problems.

Bulletin Nº65 - December 2015

IMMUNOLOGY - Fecal calprotectin as a marker of inflammatory bowel disease
The clinical suspicion of organic bowel disease motivates a diagnostic colonoscopy that generates a high rate of false positives, because the markers are not very specific. Next january, in Catlab, we will do Fecal Calprotectin (FCP), a protein found in the cytoplasm of neutrophils and in the membrane of activated monocytes and macrophages. The FCP is reliable in discriminating patients likely to suffer inflammatory bowel disease, and to detect endoscopic inflammatory activity. It is also useful to monitor response to treatment and predict the risk of recurrence.